Single Cell:Article Title: FN14 antibodies and uses thereof
Article Snippet: 100 μL of 2× HAT-containing medium (the medium described above with 2× HAT media supplement (Sigma)) was added to each well of cell suspension in 96-well plates and the plates were incubated at 37° C. with 5% CO2. .. To generate hybridomas, spleen cells from the single cell suspension were fused with mouse myeloma cells Sp2/0 (American Type Culture Collection (ATCC): CRL1581). .. Spleen cell suspensions were pelleted by centrifugation (1500 rpm, 3 min) and Red Blood Cell Lysing Buffer (Sigma) was added to the cell pellet on ice.
Suspension:Article Title: FN14 antibodies and uses thereof
Article Snippet: 100 μL of 2× HAT-containing medium (the medium described above with 2× HAT media supplement (Sigma)) was added to each well of cell suspension in 96-well plates and the plates were incubated at 37° C. with 5% CO2. .. To generate hybridomas, spleen cells from the single cell suspension were fused with mouse myeloma cells Sp2/0 (American Type Culture Collection (ATCC): CRL1581). .. Spleen cell suspensions were pelleted by centrifugation (1500 rpm, 3 min) and Red Blood Cell Lysing Buffer (Sigma) was added to the cell pellet on ice.
Isolation:Article Title: Novel monoclonal antibodies for immunodetection of AmpC β-lactamases
Article Snippet: .. Briefly, the spleen cells of immunized mouse were isolated and fused with mouse myeloma Sp2/0 (ATCC, CRL-1581) using polyethylene glycol PEG-4000 (P7306; Sigma-Aldrich, Burlington, MA, USA), and then cultivated in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 15% fetal bovine serum (FBS) and selection reagent containing hypoxanthine, aminopterin and thymidine (HAT, Sigma-Aldrich, Burlington, MA, USA, H0262). ..
Article Title: Novel monoclonal antibodies for immunodetection of AmpC β-lactamases.
Article Snippet: .. Briefly, the spleen cells of immunized mouse were isolated and fused with mouse myeloma Sp2/0 (ATCC, CRL-1581) using polyethylene glycol PEG-4000 (P7306; SigmaAldrich, Burlington, MA, USA), and then cultivated in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 15% fetal bovine serum (FBS) and selection reagent containing hypoxanthine, aminopterin and thymidine (HAT, Sigma-Aldrich, Burlington, MA, USA, H0262). ..
Modification:Article Title: Novel monoclonal antibodies for immunodetection of AmpC β-lactamases
Article Snippet: .. Briefly, the spleen cells of immunized mouse were isolated and fused with mouse myeloma Sp2/0 (ATCC, CRL-1581) using polyethylene glycol PEG-4000 (P7306; Sigma-Aldrich, Burlington, MA, USA), and then cultivated in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 15% fetal bovine serum (FBS) and selection reagent containing hypoxanthine, aminopterin and thymidine (HAT, Sigma-Aldrich, Burlington, MA, USA, H0262). ..
Article Title: Novel monoclonal antibodies for immunodetection of AmpC β-lactamases.
Article Snippet: .. Briefly, the spleen cells of immunized mouse were isolated and fused with mouse myeloma Sp2/0 (ATCC, CRL-1581) using polyethylene glycol PEG-4000 (P7306; SigmaAldrich, Burlington, MA, USA), and then cultivated in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 15% fetal bovine serum (FBS) and selection reagent containing hypoxanthine, aminopterin and thymidine (HAT, Sigma-Aldrich, Burlington, MA, USA, H0262). ..
Selection:Article Title: Novel monoclonal antibodies for immunodetection of AmpC β-lactamases
Article Snippet: .. Briefly, the spleen cells of immunized mouse were isolated and fused with mouse myeloma Sp2/0 (ATCC, CRL-1581) using polyethylene glycol PEG-4000 (P7306; Sigma-Aldrich, Burlington, MA, USA), and then cultivated in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 15% fetal bovine serum (FBS) and selection reagent containing hypoxanthine, aminopterin and thymidine (HAT, Sigma-Aldrich, Burlington, MA, USA, H0262). ..
Article Title: Novel monoclonal antibodies for immunodetection of AmpC β-lactamases.
Article Snippet: .. Briefly, the spleen cells of immunized mouse were isolated and fused with mouse myeloma Sp2/0 (ATCC, CRL-1581) using polyethylene glycol PEG-4000 (P7306; SigmaAldrich, Burlington, MA, USA), and then cultivated in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 15% fetal bovine serum (FBS) and selection reagent containing hypoxanthine, aminopterin and thymidine (HAT, Sigma-Aldrich, Burlington, MA, USA, H0262). ..
HAT Assay:Article Title: Novel monoclonal antibodies for immunodetection of AmpC β-lactamases
Article Snippet: .. Briefly, the spleen cells of immunized mouse were isolated and fused with mouse myeloma Sp2/0 (ATCC, CRL-1581) using polyethylene glycol PEG-4000 (P7306; Sigma-Aldrich, Burlington, MA, USA), and then cultivated in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 15% fetal bovine serum (FBS) and selection reagent containing hypoxanthine, aminopterin and thymidine (HAT, Sigma-Aldrich, Burlington, MA, USA, H0262). ..
Article Title: Novel monoclonal antibodies for immunodetection of AmpC β-lactamases.
Article Snippet: .. Briefly, the spleen cells of immunized mouse were isolated and fused with mouse myeloma Sp2/0 (ATCC, CRL-1581) using polyethylene glycol PEG-4000 (P7306; SigmaAldrich, Burlington, MA, USA), and then cultivated in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 15% fetal bovine serum (FBS) and selection reagent containing hypoxanthine, aminopterin and thymidine (HAT, Sigma-Aldrich, Burlington, MA, USA, H0262). ..
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